PSI - Issue 65

Polina Tyubaeva et al. / Procedia Structural Integrity 65 (2024) 290–294 Polina Tyubaeva, Ivetta Varyan, Anatoly Popov / Structural Integrity Procedia 00 (2024) 000–000

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is important to rely on a scientifically based methodology, including a systematic selection of biocompatible polymers, modifying additives that provide a unique set of properties of the polymer material (strength, ergonomics, controlled rate of bioresorption and diffusion of drugs, a high degree of surface development, antibacterial properties, and others), and protein molecules (acting as factors growth to activate and intensify wound healing). The key role is played by the development of biodegradable and biocompatible polymer matrices most suitable for the manufacture of wound-healing biomimetic materials modified with antibacterial drugs of natural and synthetic origin; the choice of the most effective approaches to the formation of polymer structures for accelerated regeneration and methods of introducing functional additives and fixing protein molecules-wound healing activators on the surface and in the structure of the material, which will allow designing wound-healing materials for personalized medicine; formalization of an approach to the creation of effective biocompatible, antimicrobial materials with adjustable performance characteristics and a controlled rate of biodegradation based on individual user parameters. The solution of these tasks will allow us to design and prepare for the introduction into production of a new class of personalized wound healing agents based on non-woven polymer materials with special properties, which is a significant result in the development of healthcare and health-saving technologies. The key objective of the work is to select the optimal polymer matrix for the development of effective personalized products for regenerative medicine based on multicomponent bioresorbable and bioactive materials capable of initiating and supporting tissue regeneration, taking into account the requirements for wound healing material. The model polymers were analyzed in the form of thin films. The films were pressed in accordance with the temperature characteristics of the initial polymers (the temperature of the plates was set at a level exceeding the melting point of the polymer by 10 °C), the pressure was 40 Atm for 1 minute. Pressing was carried out on polyamide substrates. The thickness of the films was controlled and amounted to 90-110 microns. The thermophysical characteristics and the degree of crystallinity were evaluated by Netzsch 214 Polyma differential scanning calorimetry in an argon atmosphere at a heating rate of 10 K/min in accordance with the standard method by Tyubaeva et al. (2023). Tensile strength and modulus of elasticity of the materials was determined by uniaxial stretching on a DEVOTRANS DVT GP UG breaking machine in accordance with GOST 14236-81 "Polymer films. The method of tensile testing". The average molecular weight (Mw) was estimated by gel penetrating chromatography on the Agilent PL-GP 220 Agilent Technologies device using 3 series-connected Agilent PLgel Olexis 300 columns with a volume of 7.5 ml, calibrated for polystyrene in the range 500 – 10 000 000 g/mole according to the standard. Key properties of model polymers are shown in Table 1. To analyze the potential of bioresorption of materials, model media were used: a phosphate buffer and a complex medium in the form of a soil populated with a microbiotic component. These model media can clearly demonstrate the potential for degradation as a result of hydrolysis and interaction with the microbiota. The resulting matrixes were placed in the soil to assess the loss of mass and the change in Mw. The exposure in the soil and the mass loss assessment were carried out in accordance with the methodology by Gasparyan et al. (2023). The soil temperature was 22 ± 3 °C, pH 6, humidity 60%. The exposure in the phosphate buffer and the mass loss assessment were carried out in accordance with the methodology by Kandhasamy et al. (2017). The temperature in the thermos cabinet was constantly maintained and was 37 ± 3 °C, pH 7.5. The results are shown in Table 2. 2. Materials and methods To identify factors affecting the rate of degradation of the polymer matrix for the possibility of directional regulation of bioresorption processes in a living organism, control matrixes obtained from high-density polyethylene (PE), polylactide (PLA), polycaprolactone (PCL), poly-3-hydroxybutyrate (PHB) were selected. 3. Results and discussion