PSI - Issue 33

3

Author name / Structural Integrity Procedia 00 (2019) 000–000

Ashley Amanda Freeman et al. / Procedia Structural Integrity 33 (2021) 265–278

267

Nomenclature h

Displacement, penetration depth First quartile BONE Glue made from the animal bones H Hardness d Indentation separation ISO Q1

International Organization for Standardization

IQR

Interquartile range

Load

P

P - h h max P max O-P

Load-displacement Maximum displacement

Maximum load Oliver and Pharr Reduced modulus Standard error Third quartile

E r

SE Q3

2. Materials and methods 2.1. Test specimen

4 % w/w (glue granules/water) proteinaceous adhesive films were produced by solubilising glue material in distilled water for 24 hours. After which, the solutions were heated in a double boiler and mixed until the suggested heating temperature was reached (60-65 °C). Once the solutions reached room temperature (~22 °C), 20 ml aliquots were added to Petri dishes and allowed to dry in ambient conditions for at least seven months. The process of making the adhesive films is shown in Fig. 1. See Table 1 for additional product information.

Fig. 1. Illustration of adhesive film making process; (a) commercial product, (b) closeup of granular gelatine before soaking in water, (c) granular gelatine after addition of water, (d) granular gelatine after addition of 24 immersion in water, (e) casting films, and (f) adhesive film after drying in ambient conditions.