PSI - Issue 50

E.G. Zemtsova et al. / Procedia Structural Integrity 50 (2023) 307–313 Author name / Structural Integrity Procedia 00 (2019) 000 – 000

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nanometer pores of the TiO 2 xerogel. In addition, microphotographs confirm that the resulting composite coating has no cytotoxicity and the ability of MC3T3-E1 osteoblast cells to adhere more than 85% in 72 h. As a result of the antibacterial activity study, the presence of antibacterial properties of Ag-containing samples was confirmed in comparison with samples containing only a TiO 2 layer (Table 1). Table 1. Studies of the TiO 2 /Ag composite coating on antibacterial activity Culture strains Sample composition 1. Enterococcus faecalis АТСС 29812 2. Staphylococcus aureus АТСС 25912 3. Klebsiella pneumoniae АТСС 19882 4. Acinetobacter baumannii 987 5. Pseudomonas aeruginosa ATСC 27853

Control Muller-Hinton agar

microorganisms growth microorganisms growth

microorganisms growth microorganisms growth

microorganisms growth microorganisms growth

microorganisms growth microorganisms growth

microorganisms growth microorganisms growth

Control ТiO 2

100% No growth

100% No growth

100% No growth

100% No growth

100% No growth

TiO 2 /Ag

It can be seen from the table that the contaminated control samples and control samples without silver containing only TiO 2 coating microbial growth is showed. In the TiO 2 /Ag island-type composite coating, 100% absence of culture growth is observed. As a result of the study of samples for antibacterial activity, the presence of antibacterial properties of samples containing Ag structures was confirmed in comparison with samples containing only a TiO 2 layer. The results of this study allow us to conclude that it is promising to apply an island-like composite Ag coating on Ti implants. 4. Conclusion In this work, a technique was developed for the electrochemical template synthesis of a TiO 2 /Ag composite coating by successive application of the sol-gel method using the dip-coating technology and electrochemical deposition. The resulting coatings are promising for the creation of implants, because they have a two-level relief ordering, consisting of micro- and nano-irregularities, increase the bioactivity of the implant, as they improve adhesion and promote the start of the process of osteoblast differentiation. The applied Ag layer as antibacterial agent prevents the development of infection, inflammation and, as a result, rejection of the implant, as well as reduces the engraftment period. Cytological and antibacterial studies revealed that the coatings of this composition are promising in the development of implants in dentistry and orthopedics. Funding The research was financially supported by the Russian Science Foundation grant (project No. 22-21-00573). Acknowledgements The research was carried out using the equipment of the resource centers of the St. Petersburg State University Scientific Park "X-ray Diffraction research methods" and "Nanotechnology". Conflicts of Interest The authors declare no conflict of interest.